Mo Y, Vaessen B, Johnston K, Marmorstein R
The Wistar Institute, Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA.
Mol Cell. 1998 Aug;2(2):201-12. doi: 10.1016/s1097-2765(00)80130-6.
SAP-1 is a member of the Ets transcription factors and cooperates with SRF protein to activate transcription of the c-fos protooncogene. The crystal structures of the conserved ETS domain of SAP-1 bound to DNA sequences from the E74 and c-fos promoters reveal that a set of conserved residues contact a GGA core DNA sequence. Discrimination for sequences outside this core is mediated by DNA contacts from conserved and nonconserved protein residues and sequence-dependent DNA structural properties characteristic of A-form DNA structure. Comparison with the related PU.1/DNA and GABPalpha/beta/DNA complexes provides general insights into DNA discrimination between Ets proteins. Modeling studies of a SAP-1/SRF/DNA complex suggest that SRF may modulate SAP-1 binding to DNA by interacting with its ETS domain.
SAP-1是Ets转录因子的成员之一，它与SRF蛋白协同作用以激活原癌基因c-fos的转录。与来自E74和c-fos启动子的DNA序列结合的SAP-1保守ETS结构域的晶体结构表明，一组保守残基与GGA核心DNA序列接触。对该核心以外序列的识别是由保守和非保守蛋白质残基与A-型DNA结构特有的序列依赖性DNA结构特性所形成的DNA接触介导的。与相关的PU.1/DNA和GABPα/β/DNA复合物的比较为深入了解Ets蛋白之间的DNA识别提供了思路。对SAP-1/SRF/DNA复合物的建模研究表明，SRF可能通过与其ETS结构域相互作用来调节SAP-1与DNA的结合。
