Barone E J, Yager D R, Pozez A L, Olutoye O O, Crossland M C, Diegelmann R F, Cohen I K
Wound Healing Center, Division of Plastic and Reconstructive Surgery, at the Medical College of Virginia/Virginia Commonwealth University, Richmond 23298-0117, USA.
Plast Reconstr Surg. 1998 Sep;102(4):1023-7; discussion 1028-9.
Interleukin-1-alpha (IL-1alpha) is a member of a family of proinflammatory polypeptide mediators that has been shown in vitro to stimulate collagenase production. Collagenase is a proteolytic enzyme classified as one of the matrix metalloproteinases (MMP-1) that specifically recognizes and cleaves collagen. Therefore, the objective of this study was to compare the levels of these two proteins in chronic wounds as possible factors in the pathogenesis of chronic wounds. Fluids from 10 chronic wounds were collected before and after a 1-week treatment with a hydroactive dressing (Cutinova cavity). In addition, fluids were collected from 20 acute wounds for comparison. IL-1alpha and MMP-1 levels were quantified using sandwich ELISA. Collagenase activity was measured using a radiolabeled collagen as substrate. Clinically, the chronic wounds showed decreased area (-21.0 cm2) and reduced volume (-134.5 cm3) by 4 weeks after treatment with the hydroactive dressing. There were no significant differences in the protein concentrations between acute wound fluids (21.0 +/- 3.0 mg/ml) and chronic wound fluids before and after treatment with the hydroactive dressing (18.3 +/- 5.5 and 25.2 +/- 7.6 mg/ml, respectively). Levels of IL-1alpha in the acute wound fluids were low (0.019 pg/mg), whereas in the chronic wound fluid before treatment they had been significantly elevated (44.9 + 21.8 pg/mg). Following treatment with the hydroactive dressing, the IL-1alpha levels dropped to 10.3 + 3.3 pg/mg (p < 0.05). Collagenase activity was not detectable in acute wound fluid, elevated in pretreatment chronic wounds (12.9 + 3.4 units), and decreased in chronic wounds after treatment (11.4 + 3.3 units). This study correlated clinical healing of chronic wounds with biochemical changes in the ulcer microenvironment. As the chronic wounds began to heal, there was a significant decrease in the IL-1alpha levels and collagenase activity, thus suggesting that these two proteins may contribute to the lack of healing characteristic of chronic wounds.
白细胞介素-1α（IL-1α）是促炎多肽介质家族的一员，体外实验表明它能刺激胶原酶的产生。胶原酶是一种蛋白水解酶，属于基质金属蛋白酶（MMP-1），能特异性识别并切割胶原蛋白。因此，本研究的目的是比较这两种蛋白在慢性伤口中的水平，作为慢性伤口发病机制的可能因素。在用活性水凝胶敷料（Cutinova cavity）治疗1周前后，收集了10个慢性伤口的渗出液。此外，收集了20个急性伤口的渗出液用于比较。使用夹心ELISA法定量IL-1α和MMP-1水平。以放射性标记的胶原蛋白为底物测量胶原酶活性。临床上，使用活性水凝胶敷料治疗4周后，慢性伤口面积减小（-21.0 cm²），体积减少（-134.5 cm³）。急性伤口渗出液（21.0±3.0 mg/ml）与活性水凝胶敷料治疗前后慢性伤口渗出液（分别为18.3±5.5和25.2±7.6 mg/ml）的蛋白质浓度无显著差异。急性伤口渗出液中IL-1α水平较低（0.019 pg/mg），而治疗前慢性伤口渗出液中IL-1α水平显著升高（44.9 + 21.8 pg/mg）。使用活性水凝胶敷料治疗后IL-1α水平降至10.3 + 3.3 pg/mg（p < 0.05）。急性伤口渗出液中未检测到胶原酶活性，预处理慢性伤口中胶原酶活性升高（12.9 + 3.4单位），治疗后慢性伤口中胶原酶活性降低（11.4 + 3.3单位）。本研究将慢性伤口的临床愈合与溃疡微环境中的生化变化相关联。随着慢性伤口开始愈合，IL-1α水平和胶原酶活性显著降低，因此表明这两种蛋白可能导致慢性伤口愈合不良的特征。
