Agren M S, Engel M A, Mertz P M
Department of Dermatology and Cutaneous Surgery, University of Miami School of Medicine, Florida.
Plast Reconstr Surg. 1994 Sep;94(3):518-24.
Epithelialization of second-degree burn wounds is known to be accelerated by topical treatment with hydrogel dressings and further enhanced by pulsed electrical stimulation compared with no treatment (air exposure). Tissue collagenase has been proposed to be involved during the process of epithelialization. In the present study collagenase levels were examined in partial-thickness burn wounds in the skin of four domestic pigs. Collagenase levels, assayed on postburn days 1 to 10, were substantially reduced in deblistered and air-exposed burn wounds compared with excisional partial-thickness wounds. Early application of hydrogel dressing to the burn wounds was accompanied by elevated collagenase activities and an increased inflammatory reaction in dermis. Addition of pulsed electrical stimulation increased (p < 0.001) collagenase levels twofold above those with hydrogel alone during initiation of epithelialization (postburn days 3 and 4). These results suggest that collagenase is closely linked to wound epithelialization.
已知与不进行治疗（暴露于空气中）相比，用水凝胶敷料进行局部治疗可加速二度烧伤创面的上皮化，而脉冲电刺激可进一步增强上皮化。有人提出组织胶原酶参与上皮化过程。在本研究中，检测了4头家猪皮肤中部分厚度烧伤创面的胶原酶水平。与切除的部分厚度创面相比，在烧伤后第1至10天测定的胶原酶水平在去除水疱并暴露于空气中的烧伤创面中显著降低。早期将水凝胶敷料应用于烧伤创面会伴随着胶原酶活性升高以及真皮中炎症反应增加。在开始上皮化时（烧伤后第3天和第4天），添加脉冲电刺激使胶原酶水平比单独使用水凝胶时增加了两倍（p < 0.001）。这些结果表明胶原酶与创面上皮化密切相关。
