Formation of a derivative of aflatoxin B1 on a sephadex column with a 1% aqueous solution of methanol.

A PARTIAL SEPARATION OF AFLATOXINS B1 and B2 on a Sephadex column with a 1% aqueous solution of methanol as mobile phase was achieved. The chromatographic system is, however, not neutral for aflatoxin B1. During elution a derivative--probably aflatoxin B1 hemiacetal-is produced. The derivative forms blue fluorescent spots on Adsorbosil-1 plates with Rf 0.15 when developed in chloroform/acetone (90 + 10).