一幅由CIC酵母人工染色体、P1、转化人工染色体和细菌人工染色体克隆的两个重叠群所代表的拟南芥3号染色体物理图谱。
A physical map of Arabidopsis thaliana chromosome 3 represented by two contigs of CIC YAC, P1, TAC and BAC clones.
作者信息
Sato S, Kotani H, Hayashi R, Liu Y G, Shibata D, Tabata S
机构信息
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
出版信息
DNA Res. 1998 Jun 30;5(3):163-8. doi: 10.1093/dnares/5.3.163.
We have constructed a physical map of Arabidopsis thaliana chromosome 3 by ordering the clones from CIC YAC, P1, TAC and BAC libraries using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 112 DNA markers, 145 YAC end sequences, and 156 end sequences of P1, TAC and BAC clones. The entire genome of chromosome 3, except for the centromeric and telomeric regions, was covered by two large contigs, 13.6 Mb and 9.2 Mb long. This physical map will facilitate map-based cloning experiments as well as genome sequencing of chromosome 3. The map and end sequence information are available on the KAOS (Kazusa Arabidopsis data Opening Site) web site at http://www.kazusa.or.jp/arabi/.
我们通过使用多种遗传标记和 EST 标记的序列以及克隆的末端序列，对来自 CIC YAC、P1、TAC 和 BAC 文库的克隆进行排序，构建了拟南芥 3 号染色体的物理图谱。使用的标记有 112 个 DNA 标记、145 个 YAC 末端序列以及 156 个 P1、TAC 和 BAC 克隆的末端序列。3 号染色体的整个基因组，除了着丝粒和端粒区域，被两个大的重叠群覆盖，长度分别为 13.6 Mb 和 9.2 Mb。这个物理图谱将有助于基于图谱的克隆实验以及 3 号染色体的基因组测序。该图谱和末端序列信息可在 KAOS（Kazusa 拟南芥数据开放网站）网站（http://www.kazusa.or.jp/arabi/）上获取。
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