拟南芥第5号染色体的精细物理图谱：构建序列就绪的重叠群图谱。
A fine physical map of Arabidopsis thaliana chromosome 5: construction of a sequence-ready contig map.
作者信息
Kotani H, Sato S, Fukami M, Hosouchi T, Nakazaki N, Okumura S, Wada T, Liu Y G, Shibata D, Tabata S
机构信息
Kazusa DNA Research Institute, Chiba, Japan.
出版信息
DNA Res. 1997 Dec 31;4(6):371-8. doi: 10.1093/dnares/4.6.371.
A fine physical map of Arabidopsis thaliana chromosome 5 was constructed by ordering the clones from YAC, P1, TAC and BAC libraries of the genome using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 88 genetic markers, 13 EST markers, 87 YAC end probes, 100 YAC subclone end probes, and 390 end probes of P1, TAC and BAC clones. The entire genome of chromosome 5, except for the centromeric and telomeric regions, was covered by two large contigs 11.6 Mb and 14.2 Mb long separated by the centromeric region. The minimum tiling path of the chromosome was constituted by a total of 430 P1, TAC and BAC clones. The map information is available at the Web site http://www.kazusa.or.jp/arabi/.
通过使用多种遗传标记和EST标记的序列以及克隆的末端序列，对来自基因组的YAC、P1、TAC和BAC文库的克隆进行排序，构建了拟南芥第5号染色体的精细物理图谱。所使用的标记包括88个遗传标记、13个EST标记、87个YAC末端探针、100个YAC亚克隆末端探针以及390个P1、TAC和BAC克隆的末端探针。除着丝粒和端粒区域外，第5号染色体的整个基因组被两个大的重叠群覆盖，长度分别为11.6 Mb和14.2 Mb，中间隔着着丝粒区域。该染色体的最小拼接路径由总共430个P1、TAC和BAC克隆构成。图谱信息可在网站http://www.kazusa.or.jp/arabi/上获取。
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