Peptide substrates dissolved in dimethylformamide may be modified at the epsilon-amino group of lysyl residues, causing erroneous kinetic characterization of proteolytic enzymes.
作者信息
Olesen K, Breddam K
机构信息
Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, DK-2500, Denmark.
出版信息
Anal Biochem. 1998 Aug 15;262(1):88-9. doi: 10.1006/abio.1998.2741.