来自斯氏欧文氏菌的质粒pSW200的分离与鉴定

Isolation and characterization of plasmid pSW200 from Erwinia stewartii.

作者信息

Fu J F, Hu J M, Chang Y S, Liu S T

机构信息

Department of Microbiology and Immunology, Chang-Gung University, Taoyuan, Taiwan.

出版信息

Plasmid. 1998 Sep;40(2):100-12. doi: 10.1006/plas.1998.1350.

DOI:10.1006/plas.1998.1350

PMID:9735312

Abstract

The nucleotide sequence of pSW200 of Erwinia stewartii SW2 was determined. This plasmid is 4367 bp long, consisting of four mobilization genes, mobCABD, and an origin of replication homologous to those of ColE1-type plasmids. The plasmid also contains a region of forty-one 15-bp repeats. Deleting this region does not affect the stability or the copy number when maintained as sole plasmid in the cell. However, the plasmid is rapidly lost when a homoplasmid with the intact repeat region is introduced into the cell. The function of this region may provide pSW200 an advantage in competing with an incompatible plasmid in the cell.

摘要

测定了斯氏欧文氏菌SW2的pSW200的核苷酸序列。该质粒长4367 bp，由四个转移基因mobCABD和一个与ColE1型质粒同源的复制起点组成。该质粒还包含一个由41个15 bp重复序列组成的区域。当作为细胞中的唯一质粒维持时，删除该区域不影响其稳定性或拷贝数。然而，当将具有完整重复区域的同性质粒引入细胞时，该质粒会迅速丢失。该区域的功能可能使pSW200在与细胞中不相容质粒竞争时具有优势。