Cross D P, Sang J H
J Embryol Exp Morphol. 1978 Jun;45:161-72.
A new procedure is described for the preparation of in vitro cell cultures from individual early gastrulae of Drosophila melanogaster. In these cultures several identifiable cell types differentiate within 24 h (nerve, muscle, fat-body, haemocyte and chitin-secreting); their initial appearance and continuing development over a period of weeks is described. It is proposed that this technique may be used to analyse abnormalities of cellular development in embryonic lethal mutants. Culture in vitro of cells from lethal embryos is seen to have two broad roles: (1) to test the developmental capacity of individual cell types in a situation where they are relatively free from possible deleterious interactions with other cell types and are liberated from the system of the dying embryo, and (2) through the preparation of mixed cultures from normal and mutant embryos, to determine the influence of the presence of wild-type cells on observed abnormalities of a particular cell type.
本文描述了一种从黑腹果蝇单个早期原肠胚制备体外细胞培养物的新方法。在这些培养物中，几种可识别的细胞类型在24小时内分化（神经、肌肉、脂肪体、血细胞和几丁质分泌细胞）；描述了它们在数周内的初始出现和持续发育情况。有人提出，该技术可用于分析胚胎致死突变体中细胞发育的异常情况。来自致死胚胎的细胞体外培养具有两个广泛的作用：（1）在相对不受与其他细胞类型可能有害相互作用影响且从濒死胚胎系统中解放出来的情况下，测试单个细胞类型的发育能力；（2）通过从正常和突变胚胎制备混合培养物，确定野生型细胞的存在对特定细胞类型观察到的异常情况的影响。
