Romeike B F, Feiden W
Department of Neuropathology, University Clinics of the Saarland, Homburg/Saar.
Biotech Histochem. 1998 Jul;73(4):198-201. doi: 10.3109/10520299809141110.
With current paraffin embedding techniques it is not possible to demonstrate metal particles in vascular lumens. We used a modified methyl methacrylate embedding method for obtaining plastic blocks which were cut with a precision diamond saw, and subsequently ground and polished to preserve the interface of reactive tissue surrounding platinum microcoils in arteriovenous malformations. Artifact-free interfaces could be achieved by slow embedding under vacuum conditions, slow polymerization in a water bath, and very gentle cutting, grinding and polishing techniques. The method produces good preservation of tissue and cells directly adjacent to metal coils. Our method is useful for the histomorphological interpretation of vascular pathologies in which metal particles have been introduced.
采用当前的石蜡包埋技术，无法在血管腔内显示金属颗粒。我们使用了一种改良的甲基丙烯酸甲酯包埋方法来制作塑料块，用精密金刚石锯切割这些塑料块，随后进行研磨和抛光，以保留动静脉畸形中围绕铂微线圈的反应性组织界面。通过在真空条件下缓慢包埋、在水浴中缓慢聚合以及采用非常轻柔的切割、研磨和抛光技术，可以获得无伪像的界面。该方法能很好地保存紧邻金属线圈的组织和细胞。我们的方法对于解释已引入金属颗粒的血管病变的组织形态学特征很有用。
