Ghassemifar R, Franzén L
Department of Pathology II, University Hospital, Linköping, Sweden.
Biotech Histochem. 1992 Nov;67(6):363-6. doi: 10.3109/10520299209110051.
A new double-embedding technique for thin tissue membranes is presented. This technique is useful for thin membranes such as mesenteric membranes from rodents, which usually measure only 10 microns in thickness. Several membranes are fixed and mounted on four needles located at the bottom of a plastic box. The box is filled with agarose at 50 C and then allowed to solidify. The agarose block is then removed, dehydrated in alcohol, cleared with HistoPetrol (isoparaffin hydrocarbons), permeated with paraffin and sectioned. The morphology is comparable to that obtained with methacrylate plastic embedding but is less time-consuming, less hazardous since no plastic hardener and activator are used and makes immunohistochemical studies easier.
本文介绍了一种用于薄组织膜的新型双重包埋技术。该技术适用于诸如来自啮齿动物的肠系膜膜等薄膜，其厚度通常仅为10微米。将几张膜固定并安装在位于塑料盒底部的四根针上。向盒中加入50℃的琼脂糖，然后使其凝固。接着取出琼脂糖块，在酒精中脱水，用HistoPetrol（异链烷烃）透明，用石蜡渗透并切片。其形态与用甲基丙烯酸酯塑料包埋获得的形态相当，但耗时更少，由于不使用塑料硬化剂和活化剂，危险性更低，并且使免疫组织化学研究更容易。
