Dubey I, Pratviel G, Meunier B
Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse Cedex 4, France.
Bioconjug Chem. 1998 Sep-Oct;9(5):627-32. doi: 10.1021/bc9702213.
During the preparation of a fluorescent conjugate based on a manganese cationic porphyrin carboxylate derivative linked to a 5'-amino-3'-fluorescein-labeled oligonucleotide, we observed the chemical transformation of the thiourea linkage to a guanidinium function during the deprotection in ammonia of the 3'-fluorescein-oligonucleotide from CPG support. We also noted a secondary reactivity of the activated carboxylate group of the metalloporphyrin precursor with the fluorescein entity of the conjugated oligonucleotide.
