用于RNA微螺旋结合的肽基序的功能分析表明了RNA结合结构域的新家族。
Functional analysis of peptide motif for RNA microhelix binding suggests new family of RNA-binding domains.
作者信息
Ribas de Pouplana L, Buechter D, Sardesai N Y, Schimmel P
机构信息
The Skaggs Institute for Chemical Biology and Departments of Molecular Biology and Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
EMBO J. 1998 Sep 15;17(18):5449-57. doi: 10.1093/emboj/17.18.5449.
Abstract
RNA microhelices that recreate the acceptor stems of transfer RNAs are charged with specific amino acids. Here we identify a two-helix pair in alanyl-tRNA synthetase that is required for RNA microhelix binding. A single point mutation at an absolutely conserved residue in this motif selectively disrupts RNA binding without perturbation of the catalytic site. These results, and findings of similar motifs in the proximity of the active sites of other tRNA synthetases, suggest that two-helix pairs are widespread and provide a structural framework important for contacts with bound RNA substrates.
摘要
能够重现转运RNA受体茎的RNA微螺旋被特定氨基酸负载。我们在此鉴定出丙氨酰-tRNA合成酶中一个RNA微螺旋结合所需的双螺旋对。该基序中一个绝对保守残基处的单点突变选择性地破坏RNA结合，而不干扰催化位点。这些结果，以及在其他tRNA合成酶活性位点附近发现的类似基序，表明双螺旋对广泛存在，并为与结合的RNA底物的接触提供了重要的结构框架。
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本文引用的文献
1
Reconstruction of quaternary structures of class II tRNA synthetases by rational mutagenensis of a conserved domain.
Biochemistry. 1997 Dec 9;36(49):15041-8. doi: 10.1021/bi971788+.
2
Structures of RNA-binding proteins.RNA结合蛋白的结构
Q Rev Biophys. 1997 Aug;30(3):195-240. doi: 10.1017/s0033583597003351.
3
Protein fold recognition by prediction-based threading.基于预测穿线法的蛋白质折叠识别
J Mol Biol. 1997 Jul 18;270(3):471-80. doi: 10.1006/jmbi.1997.1101.
4
Crystal structure analysis of the activation of histidine by Thermus thermophilus histidyl-tRNA synthetase.嗜热栖热菌组氨酸 - tRNA合成酶对组氨酸激活作用的晶体结构分析。
Biochemistry. 1997 Mar 18;36(11):3084-94. doi: 10.1021/bi9618373.
5
Analysis of topological and nontopological structural similarities in the PDB: new examples with old structures.蛋白质数据银行中拓扑和非拓扑结构相似性分析：旧结构的新例证
Proteins. 1996 Jul;25(3):354-65. doi: 10.1002/(SICI)1097-0134(199607)25:3<354::AID-PROT7>3.0.CO;2-F.
6
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7
Structural analysis based on state-space modeling.基于状态空间建模的结构分析。
Protein Sci. 1993 Mar;2(3):305-14. doi: 10.1002/pro.5560020302.
8
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