Chazal M, Benchimol D, Baqué P, Pierrefite V, Milano G, Bourgeon A
Service de Chirurgie Abdominale et Thoracique, Hpital de 1 Archet 11, Nice France.
Surgery. 1998 Sep;124(3):536-40.
Electrochemotherapy, which consist of local or systemic administration of a cytotoxic agent followed by application of electric pulses to a tumor, has proved effective for various types of tumors in animals and for cutaneous and head and neck cancers in human beings. This is the first study to investigate the efficacy of electrochemotherapy for treatment of hepatic metastases of colorectal cancer in the rat.
After induction of a solitary hepatic metastasis in 36 male BDIX rats, the animals were randomized to one of four groups: B-E-(no treatment), B+E-(intratumoral bleomycin), B-E+ (application of electric pulses to the tumor), and B+E+ (electrochemotherapy: intratumoral bleomycin followed by application of electric pulses).
Groups B-E and B-E+ had no tumor response. Group B+E had one partial response. Group B+E+ had seven partial responses and two complete responses. The difference in terms of response between group B+E+ and the other three groups was statistically significant (P < .05). Comparison of the mean posttherapy tumor volumes (B-E-, 50.6 mm3; B+E-, 58.7 mm3; B-E+, 46 mm3; and B+E+, 5.65 mm3) revealed a significantly smaller residual tumor in group B+E+ than in the other three groups (P < .05).
Electrochemotherapy is an effective means to reduce the volume of hepatic metastases of colorectal cancer in the rat. Additional research is required to determine the optimum treatment duration, dose effects, volume of tumor that can be treated by electrochemotherapy, and impact on survival. Such experimental studies are indispensable prerequisites for clinical trials.
电化学疗法是在局部或全身给予细胞毒性药物后，对肿瘤施加电脉冲，已被证明对动物的各种类型肿瘤以及人类的皮肤癌和头颈癌有效。这是第一项研究电化学疗法治疗大鼠结直肠癌肝转移疗效的研究。
在36只雄性BDIX大鼠中诱导出孤立性肝转移后，将动物随机分为四组之一：B-E-（不治疗）、B+E-（瘤内注射博来霉素）、B-E+（对肿瘤施加电脉冲）和B+E+（电化学疗法：瘤内注射博来霉素后施加电脉冲）。
B-E组和B-E+组无肿瘤反应。B+E组有1例部分缓解。B+E+组有7例部分缓解和2例完全缓解。B+E+组与其他三组在反应方面的差异具有统计学意义（P <.05）。比较治疗后平均肿瘤体积（B-E-，50.6 mm³；B+E-，58.7 mm³；B-E+，46 mm³；B+E+，5.65 mm³）发现，B+E+组的残余肿瘤明显小于其他三组（P <.05）。
电化学疗法是减少大鼠结直肠癌肝转移体积的有效方法。需要进一步研究以确定最佳治疗持续时间、剂量效应、可通过电化学疗法治疗的肿瘤体积以及对生存的影响。此类实验研究是临床试验必不可少的前提条件。
