Kim G T, Tsukaya H, Uchimiya H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Planta. 1998 Oct;206(2):175-83. doi: 10.1007/s004250050389.
The CURLY LEAF (CLF) gene in Arabidopsis thaliana (L). Heynh. is required for stable repression of a floral homeotic gene, AGAMOUS in leaves and stems To clarify the function of CLF in organ development, we characterized clf mutants using an anatomical and genetic approach. The clf mutants had normal roots, hypocotyls, and cotyledons, but the foliage leaves and the stems had reduced dimensions. A decrease both in the extent of cell elongation and in the number of cells was evident in the clf mutant leaves, suggesting that the CLF gene might be involved in the division and elongation of cells during leaf morphogenesis. An analysis of the development of clf mutant leaves revealed that the period during which tell division or cell elongation occurred was of normal duration, while the rates of both cell production and cell elongation were lower than in the wild type. Two phases in the elongation of cells were also recognized from this analysis. From analysis of an angustifolia clf double mutant, we found that the two phases of elongation of leaf cells were regulated independently by each gene. Thus, the CLF gene appears to affect cell division at an earlier stage and cell elongation throughout the development of leaf primordia.
拟南芥（Arabidopsis thaliana (L.) Heynh.）中的卷曲叶（CURLY LEAF，CLF）基因对于稳定抑制花同源异型基因AGAMOUS在叶片和茎中的表达是必需的。为了阐明CLF在器官发育中的功能，我们采用解剖学和遗传学方法对clf突变体进行了表征。clf突变体的根、下胚轴和子叶正常，但叶片和茎的尺寸减小。在clf突变体叶片中，细胞伸长程度和细胞数量均明显减少，这表明CLF基因可能参与叶片形态发生过程中的细胞分裂和伸长。对clf突变体叶片发育的分析表明，细胞分裂或细胞伸长发生的时期持续时间正常，而细胞产生率和细胞伸长率均低于野生型。从该分析中还识别出细胞伸长的两个阶段。通过对窄叶clf双突变体的分析，我们发现叶片细胞伸长的两个阶段由每个基因独立调控。因此，CLF基因似乎在叶原基发育的早期阶段影响细胞分裂，并在整个过程中影响细胞伸长。
