Zhao J, Carr P W
Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
Anal Chem. 1998 Sep 1;70(17):3619-28. doi: 10.1021/ac980173v.
The similarities and differences in retention characteristics of aromatic and aliphatic phases have been elucidated by the use of linear solvation energy relationships (LSERs). Three aromatic phases and three aliphatic phases were investigated in a series of mobile phases. The results of LSERs on a polymer-based aromatic phase, poly(styrene-divinylbenzene) resin (PRP-1) are very different from those on either silica- or zirconia-based aromatic and aliphatic phases. Retention on all aromatic and aliphatic phases except PRP-1 is dominated by two factors: the solute size and hydrogen bond acceptor basicity. On the other hand, in addition to these two major contributions, retention on PRP-1 is markedly influenced by the solute hydrogen bond donor acidity. We believe that PRP-1 exhibits a more adsorption-like retention mechanism than do the other phases. With the inorganic oxide-based phases, the aromatic phases are less retentive than the aliphatic phases but show a larger dependence on molecular polarizability. The enhanced polarizability of aromatic phases is the likely cause of some differences in their chromatographic selectivity relative to the aliphatic phases.
通过使用线性溶剂化能关系（LSERs），已阐明了芳香族和脂肪族固定相保留特性的异同。在一系列流动相中研究了三种芳香族固定相和三种脂肪族固定相。基于聚合物的芳香族固定相聚（苯乙烯 - 二乙烯基苯）树脂（PRP - 1）的LSERs结果与基于硅胶或氧化锆的芳香族和脂肪族固定相的结果非常不同。除PRP - 1外，所有芳香族和脂肪族固定相上的保留作用均由两个因素主导：溶质大小和氢键受体碱性。另一方面，除了这两个主要因素外，PRP - 1上的保留作用还受到溶质氢键供体酸度的显著影响。我们认为，PRP - 1比其他固定相表现出更类似吸附的保留机制。对于基于无机氧化物的固定相，芳香族固定相比脂肪族固定相的保留性弱，但对分子极化率的依赖性更大。芳香族固定相极化率的增强可能是其色谱选择性相对于脂肪族固定相存在某些差异的原因。
