Relier J P, Gamarra E, de Bethmann O, Savaglio N, Minkowski A
Arch Fr Pediatr. 1976 Feb;33(2):109-20.
Fibrinogen, factors V, II and VII + X assays have been performed by micromethod on day 1, 2 and 3 in 275 newborn infants (full-term and premature) over a period of one year -- 20 infants had proved bacterial infection (from maternal contamination) with positive cultures. The control group included 20 normal newborns in the same range of gestational age. The increase of the fibrinogen level in the infected infants appeared to be significant on day 1 and 2 but not on day 3 (Day 1: 4,06 g/l +/- 0,74 g/l versus 2,06 g/l +/- 0,28 g/l in normal newborn. p less than 0.0001; on Day 2: 4,20 +/- 0,86 g/l versus 2,32 g/l +/- 0,09 g/l p less than 0.001; on Day 3: 3,68 g/l +/- 0,62 versus 3,36 +/- 0,66 g/l). The level of fibrinogen measured in the newborn period by the micromethod was in a accordance with former works showing that it is up th the adult value after the 3rd day of life. Since the micromethod on capillary blood requires only 140 microliters of blood (whereas 2 ml with macromethod) this fibrinogen determination appears to be a rapid and reliable method for the diagnosis of neonatal infection due to materno-fetal contamination.
在一年时间里，对275名新生儿（足月儿和早产儿）在出生第1天、第2天和第3天采用微量法进行了纤维蛋白原、V因子、II因子以及VII + X因子检测。其中20名婴儿经证实有细菌感染（源自母体污染），培养结果呈阳性。对照组包括20名孕周相同的正常新生儿。感染婴儿的纤维蛋白原水平在第1天和第2天显著升高，但在第3天未出现这种情况（第1天：感染婴儿为4.06 g/l ± 0.74 g/l，正常新生儿为2.06 g/l ± 0.28 g/l，p < 0.0001；第2天：感染婴儿为4.20 ± 0.86 g/l，正常新生儿为2.32 g/l ± 0.09 g/l，p < 0.001；第3天：感染婴儿为3.68 g/l ± 0.62，正常新生儿为3.36 ± 0.66 g/l）。通过微量法在新生儿期测得的纤维蛋白原水平与之前的研究结果一致，表明出生后第3天其水平可达到成人值。由于采用微量法检测毛细血管血仅需140微升血液（而采用常量法需2毫升），因此这种纤维蛋白原检测方法对于诊断母婴污染所致的新生儿感染而言，似乎是一种快速且可靠的方法。
