近期比利时B群脑膜炎奈瑟菌分离株的分子流行病学
Molecular epidemiology of recent belgian isolates of Neisseria meningitidis serogroup B.
作者信息
Van Looveren M, Vandamme P, Hauchecorne M, Wijdooghe M, Carion F, Caugant D A, Goossens H
机构信息
Department of Microbiology, University Hospital Antwerp, UIA, Antwerp, Belgium.
出版信息
J Clin Microbiol. 1998 Oct;36(10):2828-34. doi: 10.1128/JCM.36.10.2828-2834.1998.
In Belgium an increase in the incidence of meningococcal disease has been noted since the early 1990s. Four hundred twenty clinical strains isolated during the period from 1990 to 1995, along with a set of 30 European reference strains, and 20 Dutch isolates were examined by random-primer and repetitive-motif-based PCR. A subset was investigated by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis. The data were compared with results obtained by serotyping (M. Van Looveren, F. Carion, P. Vandamme, and H. Goossens, Clin. Microbiol. Infect. 4:224-228, 1998). Both phenotypic and molecular epidemiological data suggest that the lineage III of Neisseria meningitidis, first encountered in The Netherlands in about 1980, has been introduced in Belgium. The epidemic clone, as defined by oligonucleotide D8635-primed PCR, encompasses mainly phenotypes B:4:P1.4 and B:nontypeable:P1.4, but strains with several other phenotypes were also encountered. Therefore, serotyping alone would underestimate the prevalence of the epidemic clone.
自20世纪90年代初以来，比利时已注意到脑膜炎球菌病发病率有所上升。对1990年至1995年期间分离出的420株临床菌株，以及一组30株欧洲参考菌株和20株荷兰分离株，采用随机引物和基于重复基序的聚合酶链反应（PCR）进行检测。通过多位点酶电泳和脉冲场凝胶电泳对其中一个子集进行了研究。将这些数据与血清分型结果进行比较（M. 范洛弗伦、F. 卡里翁、P. 范达默和H. 古森斯，《临床微生物学与感染》4:224 - 228，1998年）。表型和分子流行病学数据均表明，脑膜炎奈瑟菌III型菌株约于1980年首次在荷兰发现，现已传入比利时。由寡核苷酸D8635引发的PCR所定义的流行克隆，主要包括B:4:P1.4和B:不可分型:P1.4表型，但也发现了具有其他几种表型的菌株。因此，仅靠血清分型会低估流行克隆的流行率。
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