融合开始时在成肌细胞中表达的一种人类醚 - 去极化钾通道的克隆。

Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion.

作者信息

Occhiodoro T, Bernheim L, Liu J H, Bijlenga P, Sinnreich M, Bader C R, Fischer-Lougheed J

机构信息

Département de Physiologie, Centre Médical Universitaire, Hôpital Cantonal Universitaire, Switzerland.

出版信息

FEBS Lett. 1998 Aug 28;434(1-2):177-82. doi: 10.1016/s0014-5793(98)00973-9.

DOI:10.1016/s0014-5793(98)00973-9

PMID:9738473

Abstract

An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K+ current, I(K(NI)), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K+ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32-41, and is expressed as a approximately 9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I(K(NI)) indicating that h-eag constitutes the channel responsible for this current in vivo.

摘要

人类成肌细胞开始融合的一个早期迹象是一种非失活延迟整流钾电流I(K(NI))的表达以及相关的膜电位超极化。我们已经从正在分化的成肌细胞中分离出人类醚 - 去极化钾通道（h - eag）的全长编码区。h - eag基因定位于染色体1q32 - 41，并且在成肌细胞和成年脑组织中以约9 kb的转录本形式表达。在未分化的成肌细胞中强制表达h - eag会产生一种与I(K(NI))非常相似的电流，这表明h - eag构成了体内负责这种电流的通道。