Baldock P A, Morris H A, Need A G, Moore R J, Durbridge T C
Division of Clinical Chemistry, Institute of Medical and Veterinary Science, Adelaide, South Australia.
J Bone Miner Res. 1998 Sep;13(9):1451-7. doi: 10.1359/jbmr.1998.13.9.1451.
The effect of ovariectomy (OVX) on cancellous bone in the rat is not uniform at all sites of the skeleton. We report variation in the short-term effects of adult OVX in three regions of the distal femur: the diaphysis (DIA), the metaphysis (META), and the epiphysis (EPI). Cancellous bone parameters were estimated in the three separate zones of the femora and compared with changes in bone cell activity, as estimated by osteoclast surface (Oc.S) and bone formation rate (BFR). Changes were studied for 30 days in a series of rats either sham-operated (Sham) or ovariectomized (OVX) at 7 months of age. Oc.S and BFR were elevated following OVX in all regions. The time course for the OVX-induced changes differed between regions: DIA, both Oc.S and BFR were elevated at day 9; META, Oc.S was also elevated at day 9, while the rise in BFR was delayed until day 21; EPI, Oc.S remained stable but increased relative to ovary-intact rats by day 18 due to reduced levels in the latter, but BFR did not rise until day 28. These changes in bone cell activity following OVX produced a 71% reduction of cancellous bone in the DIA and a 35% reduction in the META. In contrast, no OVX-induced bone loss was observed in the EPI. This study shows that bone cell activity increases in each region of the distal femur within the first 30 days following OVX, independent of bone loss. However, the time course of increased bone cell activity is not uniform. These data highlight the role of local factors in the response to ovarian hormone deficiency.
卵巢切除术（OVX）对大鼠松质骨的影响在骨骼的所有部位并不一致。我们报告了成年OVX对股骨远端三个区域的短期影响的差异：骨干（DIA）、干骺端（META）和骨骺（EPI）。在股骨的三个独立区域估计松质骨参数，并与通过破骨细胞表面（Oc.S）和骨形成率（BFR）估计的骨细胞活性变化进行比较。在一系列7个月大时接受假手术（Sham）或卵巢切除（OVX）的大鼠中，对变化进行了30天的研究。OVX后所有区域的Oc.S和BFR均升高。OVX诱导的变化的时间进程在不同区域有所不同：在DIA，Oc.S和BFR在第9天均升高；在META，Oc.S在第9天也升高，而BFR的升高延迟至第21天；在EPI，Oc.S保持稳定，但由于卵巢完整大鼠的水平降低，到第18天相对于它们增加，但BFR直到第28天才升高。OVX后骨细胞活性的这些变化导致DIA的松质骨减少71%，META减少35%。相比之下，在EPI未观察到OVX诱导的骨质流失。这项研究表明，OVX后30天内股骨远端的每个区域骨细胞活性均增加，与骨质流失无关。然而，骨细胞活性增加的时间进程并不一致。这些数据突出了局部因素在对卵巢激素缺乏反应中的作用。
