与成人中性粒细胞相比，脐带血中性粒细胞中的自发性凋亡和Fas介导的凋亡减少。

Spontaneous and Fas-mediated apoptosis are diminished in umbilical cord blood neutrophils compared with adult neutrophils.

作者信息

Allgaier B, Shi M, Luo D, Koenig J M

机构信息

Neonatology and Leukocyte Biology, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA.

出版信息

J Leukoc Biol. 1998 Sep;64(3):331-6. doi: 10.1002/jlb.64.3.331.

DOI:10.1002/jlb.64.3.331

PMID:9738659

Abstract

Apoptosis mediates neutrophil (PMN) phagocytosis and is influenced by cytokines and the Fas/Fas ligand pathway. To determine whether apoptosis of cord blood PMN differs from those of adults, cultured PMN were evaluated by morphological analysis, flow cytometry (TUNEL assay), and DNA gel electrophoresis. In addition, we studied the effect of anti-Fas IgM or cycloheximide on induction of PMN apoptosis. Spontaneous apoptosis (24 h) was less in cord blood PMN (mean +/- SD; 29 +/- 9 vs. adults, 56 +/- 14%, P < 0.001). Treatment (6 h) with anti-Fas IgM induced less apoptosis in cord blood PMN (24 +/- 6 vs. adults, 63 +/- 7%, P < 0.001), as did treatment with cycloheximide (13 +/- 10 vs. adults, 55 +/- 16%, P < 0.01). These data suggest the pre-existence of proteins that inhibit apoptosis or the absence of those that promote apoptosis in cord blood PMN.

摘要

细胞凋亡介导中性粒细胞（PMN）的吞噬作用，并受细胞因子和Fas/Fas配体途径的影响。为了确定脐血PMN的细胞凋亡是否与成人不同，通过形态学分析、流式细胞术（TUNEL检测）和DNA凝胶电泳对培养的PMN进行了评估。此外，我们研究了抗Fas IgM或环己酰亚胺对PMN细胞凋亡诱导的影响。脐血PMN的自发凋亡（24小时）较少（平均值±标准差；29±9%，而成人是56±14%，P<0.001）。用抗Fas IgM处理（6小时）诱导脐血PMN凋亡较少（24±6%，而成人是63±7%，P<0.001），用环己酰亚胺处理也是如此（13±10%，而成人是55±16%，P<0.01）。这些数据表明脐血PMN中存在抑制细胞凋亡的蛋白质，或者缺乏促进细胞凋亡的蛋白质。