P22 Erf蛋白和宿主RecA为质粒携带重复序列的转导分离提供了替代功能。

The P22 Erf protein and host RecA provide alternative functions for transductional segregation of plasmid-borne duplications.

作者信息

Garzón A, Cano D A, Casadesús J

机构信息

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.

出版信息

Mol Gen Genet. 1998 Jul;259(1):39-45. doi: 10.1007/s004380050786.

DOI:10.1007/s004380050786

PMID:9738878

Abstract

A tandem DNA duplication carried on a ColE1-derived plasmid segregates at high frequency upon generalized transduction by phage P22 HT. Transductional segregation of the plasmid-borne duplication can be promoted either by RecA or by the Erf function of P22, indicating that transductional segregation is a consequence of the recombination events that re-circularize the plasmid in the recipient cell. RecA-mediated and Erf-mediated transduction give similar frequencies of duplication segregation and yield the same types of segregation products, indicating that two distinct recombination machineries (RecA + RecBCD and Erf + RecBCD) perform similar or identical recombination reactions on plasmid DNA substrates transduced by bacteriophage P22 HT.

摘要

携带在源自ColE1的质粒上的串联DNA重复序列，在噬菌体P22 HT进行的普遍性转导过程中会以高频发生分离。质粒携带的重复序列的转导分离可由RecA或P22的Erf功能促进，这表明转导分离是受体细胞中使质粒重新环化的重组事件的结果。RecA介导的和Erf介导的转导产生相似的重复序列分离频率，并产生相同类型的分离产物，这表明两种不同的重组机制（RecA + RecBCD和Erf + RecBCD）对噬菌体P22 HT转导的质粒DNA底物进行相似或相同的重组反应。