Garzón A, Cano D A, Casadesús J
Departamento de Genética, Universidad de Servilla, Spain.
Genetics. 1995 Jun;140(2):427-34. doi: 10.1093/genetics/140.2.427.
In the absence of host RecA function, plasmid transduction by bacteriophage P22 can be mediated by Erf recombinase. Erf is not carried on the infecting particle but synthesized upon infection. In the recipient cell, Erf can promote both generalized plasmid transduction (which requires the circularization of plasmids transduced as linear multimers) and specialized plasmid transduction (which requires the release of plasmid DNA from linear plasmid-phage cointegrates). Both processes of Erf-mediated plasmid transduction require host RecBCD function. In contrast, RecBCD is not required for Erf-mediated circularization of P22 DNA.
在宿主RecA功能缺失的情况下,噬菌体P22介导的质粒转导可由Erf重组酶介导。Erf并不存在于感染颗粒中,而是在感染时合成。在受体细胞中,Erf既能促进广义质粒转导(这需要将作为线性多聚体转导的质粒环化),也能促进特异性质粒转导(这需要从线性质粒-噬菌体共整合体中释放质粒DNA)。Erf介导的质粒转导的这两个过程都需要宿主RecBCD功能。相比之下,Erf介导的P22 DNA环化不需要RecBCD。