人多药耐药相关蛋白3（MRP3）的cDNA克隆及诱导表达

cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3).

作者信息

Kiuchi Y, Suzuki H, Hirohashi T, Tyson C A, Sugiyama Y

机构信息

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Japan.

出版信息

FEBS Lett. 1998 Aug 14;433(1-2):149-52. doi: 10.1016/s0014-5793(98)00899-0.

DOI:10.1016/s0014-5793(98)00899-0

PMID:9738950

Abstract

Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestine and colon; hepatic expression of MRP3 was observed in humans but not in normal rats. In HepG2 cells, the expression of MRP3 was induced by phenobarbital. These results suggest that MRP3 may act as an inducible transporter in the biliary and intestinal excretion of organic anions.

摘要

此前，我们克隆了大鼠多药耐药相关蛋白3（MRP3），作为有机阴离子胆汁排泄诱导转运体的候选蛋白[Hirohashi等人（1998年），《分子药理学》53卷，第1068 - 1075页]。在本研究中，我们从人结肠腺癌细胞（Caco - 2细胞）中克隆了人MRP3（1527个氨基酸）。人MRP3主要在肝脏、小肠和结肠中表达；在人类肝脏中观察到了MRP3的表达，但在正常大鼠中未观察到。在人肝癌细胞（HepG2细胞）中，苯巴比妥可诱导MRP3的表达。这些结果表明，MRP3可能在有机阴离子的胆汁和肠道排泄中作为诱导转运体发挥作用。