通过丝裂霉素C处理干酪乳杆菌诱导产生的具有粘性末端的温和噬菌体。

A temperate phage with cohesive ends induced by mitomycin C treatment of Lactobacillus casei.

作者信息

Nakashima Y, Hasuwa H, Kakita Y, Murata K, Kuroiwa A, Miake F, Watanabe K

机构信息

Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.

出版信息

Arch Virol. 1998;143(8):1621-6. doi: 10.1007/s007050050403.

DOI:10.1007/s007050050403

PMID:9739339

Abstract

A temperate phage, named PL-2, was induced from Lactobacillus casei ATCC 27092 by mitomycin C treatment of the cells at exponential growth phase. The phage had an isometric head of 45 nm in diameter and a flexible, non-contractile tail, 150 nm long and 10 nm wide, with a sharp tip. Along the tail axis, about 40 regularly spaced striae were seen. The phage DNA had complementary cohesive ends. The restriction enzyme map of the DNA was constructed by using 13 different restriction endonucleases. The size of the DNA was 35.2 kb, 83% in size of that of phage PL-1 lytic for the same Lb. casei strain.

摘要

一种名为PL-2的温和噬菌体，是通过在指数生长期用丝裂霉素C处理干酪乳杆菌ATCC 27092细胞诱导产生的。该噬菌体具有直径为45 nm的等轴头和一条灵活的、不可收缩的尾，尾长150 nm，宽10 nm，尾尖尖锐。沿尾轴可见约40条规则间隔的条纹。噬菌体DNA具有互补的粘性末端。通过使用13种不同的限制性内切酶构建了DNA的限制性酶切图谱。DNA的大小为35.2 kb，是对同一干酪乳杆菌菌株具有裂解性的噬菌体PL-1大小的83%。