Facco C, La Rosa S, Dionigi A, Uccella S, Riva C, Capella C
Department of Clinical and Biological Sciences, II Faculty of Medicine, University of Pavia at Varese, Italy.
Arch Pathol Lab Med. 1998 Sep;122(9):828-32.
Ovarian metastatic carcinoids are rare neoplasms that show prominent fibrosis of tumor stroma and are often associated with peritoneal carcinomatosis. We studied formalin-fixed and paraffin-embedded tumor specimens of 2 cases of ovarian metastases from ileal enterochromaffin cell carcinoids immunohistochemically to evaluate whether acidic fibroblast growth factor (aFGF), transforming growth factor-alpha (TGFalpha), and their respective receptors (fibroblast growth factor receptor-4 [FGFR4] and epidermal growth factor receptor [EGFR]) may play a role in the pathogenesis of stromal fibroblast reaction and in the mechanism of tumor dissemination.
In both cases, the majority of tumor cells expressed immunoreactivity for aFGF, FGFR4, and TGFalpha. Immunoreactivity for FGFR4 was detected in stromal cells of both cases, while EGFR-positive stromal cells were found in only 1 case. Immunoreactivity for FGFR4 was also found in peritoneal mesothelial cells.
The coexpression of aFGF and FGFR4 in neoplastic enterochromaffin cells suggests that aFGF may act as an autocrine factor stimulating tumor cell growth. In addition, aFGF and TGFalpha may stimulate, in a paracrine fashion, the proliferation of FGFR4- and EGFR-immunoreactive stromal fibroblasts. Finally, interaction of aFGF-immunoreactive enterochromaffin cells with FGFR4-bearing mesothelial cells may play a role in the mechanism of serosal implant and spread of tumor cells.
卵巢转移性类癌是一种罕见的肿瘤，其肿瘤基质有明显纤维化，且常与腹膜癌病相关。我们对2例回肠嗜铬细胞类癌卵巢转移的福尔马林固定石蜡包埋肿瘤标本进行免疫组化研究，以评估酸性成纤维细胞生长因子（aFGF）、转化生长因子-α（TGFα）及其各自的受体（成纤维细胞生长因子受体-4 [FGFR4]和表皮生长因子受体[EGFR]）是否可能在基质成纤维细胞反应的发病机制及肿瘤播散机制中发挥作用。
在这2例病例中，大多数肿瘤细胞对aFGF、FGFR4和TGFα呈免疫反应性。2例病例的基质细胞均检测到FGFR4免疫反应性，而仅1例发现EGFR阳性基质细胞。在腹膜间皮细胞中也发现了FGFR4免疫反应性。
aFGF和FGFR4在肿瘤嗜铬细胞中的共表达提示aFGF可能作为一种自分泌因子刺激肿瘤细胞生长。此外，aFGF和TGFα可能以旁分泌方式刺激FGFR4和EGFR免疫反应性基质成纤维细胞的增殖。最后，aFGF免疫反应性嗜铬细胞与携带FGFR4的间皮细胞之间的相互作用可能在肿瘤细胞浆膜种植和播散机制中发挥作用。
