Genton B, Paget S, Beck H P, Gibson N, Alpers M P, Hii J
Papua New Guinea Institute of Medical Research, Madang.
Southeast Asian J Trop Med Public Health. 1998 Mar;29(1):35-40.
Rapid, simple, accurate and cheap methods are required for the diagnosis of malaria in endemic areas. The ParaSight(R)-F test, which is based on qualitative detection by monoclonal antibody of the Plasmodium falciparum (Pf) histidin-rich protein-II in the blood, showed promising results. As some antigens of Pf have been detected in the urine, we assessed the efficiency of the ParaSight(R)-F test in the whole blood and in the urine using microscopy and PCR as gold standards. One hundred and twelve children living in the East Sepik Province of Papua New Guinea (PNG) were recruited during a cross-sectional community survey. When using microscopy as reference, the ParaSight(R)-F test applied to whole blood had 84% sensitivity and 77% specificity. The semi-quantitative assessment showed that the intensity of the color on the wick correlated with parasite density. The ParaSight(R)-F test performed on urine had 81% sensitivity but only 26% specificity. Very similar results for blood and urine were obtained when using PCR as reference. The present evaluation of the ParaSight(R)-F test applied to blood compares well with findings in endemic areas of Africa or Asia, and confirms its usefulness to diagnose Pf infection in endemic areas of the South Pacific. Because of the lack of specificity, the ParaSight(R)-F test performed on urine cannot be recommended.
在疟疾流行地区，需要快速、简便、准确且廉价的疟疾诊断方法。基于单克隆抗体定性检测血液中恶性疟原虫（Pf）富含组氨酸蛋白II的ParaSight(R)-F检测显示出良好的结果。由于在尿液中检测到了一些Pf抗原，我们以显微镜检查和PCR作为金标准，评估了ParaSight(R)-F检测在全血和尿液中的效率。在一项横断面社区调查中，招募了巴布亚新几内亚（PNG）东塞皮克省的112名儿童。以显微镜检查为参考时，应用于全血的ParaSight(R)-F检测灵敏度为84%，特异性为77%。半定量评估表明，试纸上颜色的强度与寄生虫密度相关。对尿液进行的ParaSight(R)-F检测灵敏度为81%，但特异性仅为26%。以PCR为参考时，血液和尿液得到了非常相似的结果。目前对应用于血液的ParaSight(R)-F检测的评估与非洲或亚洲流行地区的研究结果相当，并证实了其在南太平洋流行地区诊断Pf感染的有用性。由于缺乏特异性，不推荐对尿液进行ParaSight(R)-F检测。
