Liu J Y, Shyu J C, Chang C L, Tsai C C, Chang A C, Yang L C, Lin L Y, Hsieh Y S
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan, ROC.
Life Sci. 1998;63(9):721-30. doi: 10.1016/s0024-3205(98)00327-0.
In this study, we determined the expression of protein kinase C (PKC) isoforms during trauma-induced decidualization. The findings revealed that at least five PKC isoforms (alpha, delta, zeta, iota and lambda) were present in both control and decidualized tissues. After trauma-stimulation, PKC alpha was down-modulated in the deciduomata but not in the myometrium. Down-modulation was compatible with the increase in cell mitosis which reached a maximum at 2-3 days. On the other hand, PKC zeta was not down-modulated. It was increased both in the deciduomata and myometrium, and paralleled the frequency of decidual cell mitosis. The PKC isoforms of delta, iota and lambda were also increased, but they were associated with the depression of cell mitosis. Therefore, these findings suggested that the variable expression of PKC isoforms in trauma-induced decidualizing tissue in pseudopregnant rats may be involved in the modulation of decidual cell growth.
在本研究中，我们测定了创伤诱导蜕膜化过程中蛋白激酶C（PKC）亚型的表达。研究结果显示，对照组织和蜕膜化组织中均存在至少五种PKC亚型（α、δ、ζ、ι和λ）。创伤刺激后，蜕膜中PKCα下调，而子宫肌层中未下调。下调与细胞有丝分裂增加相一致，有丝分裂在2 - 3天达到峰值。另一方面，PKCζ未下调。它在蜕膜和子宫肌层中均增加，并与蜕膜细胞有丝分裂频率平行。PKCδ、ι和λ亚型也增加，但它们与细胞有丝分裂的抑制有关。因此，这些发现表明，假孕大鼠创伤诱导蜕膜化组织中PKC亚型的可变表达可能参与了蜕膜细胞生长的调节。
