Hoshiai H, Uehara S, Suzuki M, Villee C A
Tohoku J Exp Med. 1982 Aug;137(4):349-60. doi: 10.1620/tjem.137.349.
The present study was done to define the conditions of implantation of fertilized ovum from serial day-to-day changes in the tissue weight of deciduomata in pseudopregnancy, estrogen receptor (ER), progesterone receptor (PR), cAMP content, RNA synthesizing capacity and ornithine decarboxylase activity (ODC). Pseudopregnancy was induced in Wistar rats in the estrus morning by vaginocervical stimulation. At the 4th day of pseudopregnancy deciduomata was induced by intraluminal instillation of olive oil with simultaneous oophorectomy and intramuscular injection of 20 mg progesterone depot (the day of induction of decidualization was arbitrarily expressed as D0 and the ensuing days, as D1, D2 and so on). The number of binding sites of ER and PR was estimated by exchange assay using dextran charcoal. cAMP content and RNA synthesizing capacity were determined by RIA and 3H-UTP uptake, respectively. ODC activity was measured by the method of Jänne and Williams-Ashman. Following the induction of deciduomata the uterus weight (unilateral) increased abruptly to 2-fold at D2 and approximately 30-fold (4.5 g) at D8 and declined thereafter. ER diminished in a linear fashion from 2.77 x 10(-10) M at D2 to 0.53 x 10(-10) M at D7. On the other hand, PR was estimated 2.9 x 10(-10) at D2. 2.5 x 10(-10) M at D5 and decreased further at D6 and D7, cAMP was 77 nmoles/mg protein at D2 and decreased rapidly from D3 onward. RNA synthesizing capacity declined in a linear fashion from D3 to 1/2 to D5 and 1/3 at D7. ODC was found 1.7 CO2 nmoles/mg protein at D2, decreased at D3 and D4, but remained nearly the same at D4 and D5.
本研究旨在通过假孕期间蜕膜组织重量的逐日变化、雌激素受体（ER）、孕激素受体（PR）、环磷酸腺苷（cAMP）含量、RNA合成能力和鸟氨酸脱羧酶活性（ODC）来确定受精卵着床的条件。在发情期早晨，通过阴道宫颈刺激诱导Wistar大鼠假孕。在假孕第4天，通过向子宫腔内注入橄榄油同时进行卵巢切除术，并肌肉注射20mg长效孕酮来诱导蜕膜形成（蜕膜化诱导日任意表示为D0，随后的日子表示为D1、D2等）。ER和PR的结合位点数量通过葡聚糖活性炭交换分析法进行估计。cAMP含量和RNA合成能力分别通过放射免疫分析法（RIA）和3H-UTP摄取法测定。ODC活性通过Jänne和Williams-Ashman的方法进行测量。诱导蜕膜形成后，子宫重量（单侧）在D2时突然增加到2倍，在D8时增加到约30倍（4.5g），此后下降。ER从D2时的2.77×10⁻¹⁰M呈线性下降至D7时的0.53×10⁻¹⁰M。另一方面，PR在D2时估计为2.9×10⁻¹⁰，在D5时为2.5×10⁻¹⁰M，在D6和D7时进一步下降，cAMP在D2时为77nmol/mg蛋白质，从D3开始迅速下降。RNA合成能力从D3到D5呈线性下降至1/2，在D7时为1/3。ODC在D2时为1.7nmol CO₂/mg蛋白质，在D3和D4时下降，但在D4和D5时几乎保持不变。
