Laurent A, Rouillac C, Delezoide A L, Giovangrandi Y, Vekemans M, Bellet D, Abitbol M, Vidaud M
Laboratoire de Génétique Moléculaire, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, France.
Mol Reprod Dev. 1998 Oct;51(2):123-9. doi: 10.1002/(SICI)1098-2795(199810)51:2<123::AID-MRD1>3.0.CO;2-S.
Polypeptide growth factors play an important role in the regulation of human embryonic development. Insulin-like 4 gene (INSL4) is a member of the insulin family, which includes insulin, IGF-I, IGF-II, relaxin, and INSL3. Using RT-PCR, we previously found abundant INSL4 mRNA in the human placenta. In this study, we examined the chronology and spatial expression of this gene in sections of human placenta and conceptus by means of in situ hybridization. Expression of the IGF-II gene was studied as a positive control. INSL4 distribution was tissue- and cell-specific. Indeed, INSL4 mRNA was most abundant in syncytiotrophoblast cells. In fetal tissues, INSL4 mRNA was identified in the perichondrium of all four limbs, vertebrae, and ribs. Moreover, INSL4 mRNA was abundant in interbone ligaments. These findings indicate that the INSL4 gene may play an important role in trophoblast development and regulation of bone formation. IGF-II mRNA, in agreement with the literature, are mainly located in the mesodermal core in the villous trophoblast and in most embryonic tissues.
多肽生长因子在人类胚胎发育的调节中发挥着重要作用。胰岛素样4基因（INSL4）是胰岛素家族的成员之一，该家族包括胰岛素、胰岛素样生长因子-I（IGF-I）、胰岛素样生长因子-II（IGF-II）、松弛素和胰岛素样肽3（INSL3）。我们之前通过逆转录聚合酶链反应（RT-PCR）发现人胎盘中存在大量INSL4信使核糖核酸（mRNA）。在本研究中，我们通过原位杂交技术检测了该基因在人胎盘和孕体切片中的时间顺序及空间表达情况。以IGF-II基因的表达作为阳性对照进行研究。INSL4的分布具有组织和细胞特异性。事实上，INSL4 mRNA在合体滋养层细胞中最为丰富。在胎儿组织中，在四肢、椎骨和肋骨的软骨膜中均鉴定出了INSL4 mRNA。此外，INSL4 mRNA在骨间韧带中含量丰富。这些发现表明，INSL4基因可能在滋养层发育和骨形成调节中发挥重要作用。与文献一致，IGF-II mRNA主要位于绒毛滋养层的中胚层核心以及大多数胚胎组织中。
