Halliday J W, Powell L W, Mack U
Br J Haematol. 1976 Oct;34(2):237-50. doi: 10.1111/j.1365-2141.1976.tb00194.x.
The biological relevance of four iron-containing fractions previously detected in rat intestinal mucosal cells has been studied. The distribution of iron in these fractions obtained by chromatography on Sepharose 6B has been examined after in vivo and in vitro incubation of mucosal cells with 59Ce. In addition, the effects of phenobarbitone, cycloheximide, iron-deficiency and iron-loading on the uptake and distrubution of iron within the four mucosal cell fractions was studied. The iron in fraction I was mostly bound to intracellular membrane particles. Fraction II was shown to be ferritin. Fraction III contained some transferrin and also a protein of molecular weight similar to transferrin but which was not precipitable by antitransferrin antiserum. Quantited with the results of 'chaser' experiments suggested that, in addition to ferritin, at least two of the fractions (I and III) were involved in the process of iron absorption by the mucosal cell.
已对先前在大鼠肠黏膜细胞中检测到的四种含铁组分的生物学相关性进行了研究。在用59Ce对黏膜细胞进行体内和体外孵育后，检查了通过Sepharose 6B色谱法获得的这些组分中铁的分布。此外，还研究了苯巴比妥、环己酰亚胺、缺铁和铁过载对四种黏膜细胞组分中铁摄取和分布的影响。组分I中的铁主要与细胞内膜颗粒结合。组分II显示为铁蛋白。组分III含有一些转铁蛋白，还含有一种分子量与转铁蛋白相似但不能被抗转铁蛋白抗血清沉淀的蛋白质。“追踪”实验结果的定量分析表明，除铁蛋白外，至少其中两个组分（I和III）参与了黏膜细胞的铁吸收过程。
