Amarger V, Gauguier D, Yerle M, Apiou F, Pinton P, Giraudeau F, Monfouilloux S, Lathrop M, Dutrillaux B, Buard J, Vergnaud G
Laboratoire de Recherche en Génétique des Espèces, Institut de Biologie des Hôpitaux de Nantes, 9, Quai Moncousu, Nantes Cedex, 44035, France.
Genomics. 1998 Aug 15;52(1):62-71. doi: 10.1006/geno.1998.5365.
We have developed approaches for the cloning of minisatellites from total genomic libraries and applied these approaches to the human, rat, and pig genomes. The chromosomal distribution of minisatellites in the three genomes is strikingly different, with clustering at chromosome ends in human, a seemingly almost even distribution in rat, and an intermediate situation in pig. A closer analysis, however, reveals that interstitial sites in pig and rat often correspond to terminal cytogenetic bands in human. This observation suggests that minisatellites are created toward chromosome ends and their internalization represents secondary events resulting from rearrangements involving chromosome ends.
我们已经开发出了从全基因组文库中克隆微卫星的方法，并将这些方法应用于人类、大鼠和猪的基因组。微卫星在这三个基因组中的染色体分布显著不同，在人类中聚集在染色体末端，在大鼠中似乎几乎均匀分布，而在猪中则处于中间状态。然而，进一步分析表明，猪和大鼠中的间质位点通常对应于人类的末端细胞遗传学带。这一观察结果表明，微卫星是朝着染色体末端产生的，它们的内化代表了涉及染色体末端重排的次生事件。
