Akesson B, Arner A, Sundler R
Biochim Biophys Acta. 1976 Sep 27;441(3):453-64. doi: 10.1016/0005-2760(76)90242-3.
The 1-[3H] palmitoyl, 2-[3H] oleoyl, and 2-[14C] linoleoyl derivatives of sn-glycero-3-phosphoethanolamine and the corresponding derivatives of sn-glycero-3-phosphocholine were injected intraportally to rats and their incorporation into liver lipids was studied 15 min thereafter. Both the uptake by the liver and the degree of acylation was higher for the unsaturated compounds. The uptake of lysophosphatidylethanolamine was higher than that of lysophosphatidlycholine. The metabolism of 1-lysophosphatidylethanolamine was also studied in isolated hepatocytes. The degree of hydrolysis was much more prominent than in vivo. After injecting 2-[14C] linoleoyl derivatives, a large part of the 14C was recovered in the dienoic phospholipids. Subfractionation by reversed-phase partition chromatography showed that the isotope was located in the palmitoyllinoleoyl and stearoyl-linoleoyl fraction. The 100 X stearoly/(palmitoyl + stearoyl) ratio was 84 in dienoic phosphatidylethanolamine and 59 in dienoic phosphatidylcholine. This preference for stearic acid is significantly larger than in other pathways yielding dienoic phospholipids. It can be concluded that the monoacylphospholipid acyltransferase reactions operating at positions 1 or 2 yield different saturated acyl chain profiles in phosphatidylethanolamine and phosphatidylcholine of a specific unsaturation. This may be important in the regulation of the fatty acid composition of the membrane phospholipids.
将sn-甘油-3-磷酸乙醇胺的1-[³H]棕榈酰基、2-[³H]油酰基和2-[¹⁴C]亚油酰基衍生物以及sn-甘油-3-磷酸胆碱的相应衍生物经门静脉注射给大鼠，并在15分钟后研究它们掺入肝脏脂质的情况。不饱和化合物的肝脏摄取量和酰化程度都更高。溶血磷脂酰乙醇胺的摄取量高于溶血磷脂酰胆碱。还在分离的肝细胞中研究了1-溶血磷脂酰乙醇胺的代谢。水解程度比体内明显得多。注射2-[¹⁴C]亚油酰基衍生物后，大部分¹⁴C在二烯磷脂中回收。通过反相分配色谱进行的亚分级分离表明，同位素位于棕榈酰亚油酰基和硬脂酰亚油酰基部分。在二烯磷脂酰乙醇胺中，100×硬脂酰基/（棕榈酰基 + 硬脂酰基）的比值为84，在二烯磷脂酰胆碱中为59。这种对硬脂酸的偏好明显大于产生二烯磷脂的其他途径。可以得出结论，在1位或2位起作用的单酰基磷脂酰转移酶反应在特定不饱和度的磷脂酰乙醇胺和磷脂酰胆碱中产生不同的饱和酰基链谱。这可能对膜磷脂脂肪酸组成的调节很重要。
