Galzigna L, Garbin L, Pagura C
Biochim Biophys Acta. 1976 Oct 4;447(2):230-7. doi: 10.1016/0005-2787(76)90345-2.
A soluble RNA with a molecular weight between 15 000 and 23 000 was extracted from calf thymus and chromatographically purified. The RNA was slightly soluble in doxane (approx. 2.5 mug/ml) and RNA-dioxane solutions were used to fill a cell (452.02 pF capacitance) to which static electric fields of variable strength were applied. The dielectric permittivity of the solutions was measured at a fixed time interval after the application of the electric field. The RNA solutions showed a dielectric increment proportional to the strength of the applied field and to the RNA concentration. The existence of a hystersis effect was proven and the effect of the electric field on the RNA molecule might be related to a dielectric saturation phenomenon parallel to long-term changes of the molecule.
从牛胸腺中提取出分子量在15000至23000之间的可溶性RNA，并通过色谱法进行纯化。该RNA微溶于二氧六环（约2.5微克/毫升），使用RNA - 二氧六环溶液填充一个细胞（电容为452.02皮法），对其施加可变强度的静电场。在施加电场后的固定时间间隔测量溶液的介电常数。RNA溶液显示出与施加场强和RNA浓度成正比的介电增量。证实了滞后效应的存在，并且电场对RNA分子的影响可能与分子长期变化平行的介电饱和现象有关。
