Tautz N, Meyers G, Thiel H J
Institut für Virologie (FB Veterinärmedizin), Justus-Liebeg-Universität Giessen, Germany.
Clin Diagn Virol. 1998 Jul 15;10(2-3):121-7. doi: 10.1016/s0928-0197(98)00037-3.
Two biotypes of pestiviruses, cytopathogenic (cp) and non-cytopathogenic (noncp) viruses, are distinguished by their effects on tissue culture cells. In contrast to the bovine viral diarrhoea virus (BVDV) system, only a few cp border disease virus (BDV) and cp classical swine fever virus (CSFV) strains have been described. Antigenically closely related noncp and cp BVDV can be isolated from cattle with fatal mucosal disease (MD) and are called a virus pair. The generation of cp BVDV in an animal persistently infected with noncp BVDV is regarded as causative for the development of MD.
To analyse viral pairs of BVDV at the molecular level and thereby identify differences between the viruses of each pair.
BVDV pairs were isolated from several animals coming down with MD; the genomes of the respective BVD viruses were sequenced on cDNA level. Studies concerning the polyprotein processing of each strain were carried out.
Molecular analysis of BVDV pairs demonstrated a linkage between RNA recombination, generation of NS3 and the onset of fatal MD.
The molecular analysis of BVDV pairs revealed that the respective cp strains arise by RNA recombination from noncp viruses.
瘟病毒有两种生物型，即致细胞病变（cp）病毒和非致细胞病变（noncp）病毒，可根据它们对组织培养细胞的影响来区分。与牛病毒性腹泻病毒（BVDV）系统不同，仅描述了少数cp边境病病毒（BDV）和cp经典猪瘟病毒（CSFV）毒株。抗原性密切相关的noncp和cp BVDV可从患有致命黏膜病（MD）的牛中分离得到，它们被称为病毒对。在持续感染noncp BVDV的动物中产生cp BVDV被认为是MD发生的原因。
在分子水平上分析BVDV病毒对，从而确定每对病毒之间的差异。
从几头患有MD的动物中分离出BVDV病毒对；在cDNA水平上对相应BVD病毒的基因组进行测序。对每个毒株的多蛋白加工进行了研究。
对BVDV病毒对的分子分析表明，RNA重组、NS3的产生与致命MD的发生之间存在联系。
对BVDV病毒对的分子分析表明，相应的cp毒株是由noncp病毒通过RNA重组产生的。
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