Martínez M, Vayá A, Server R, Gilsanz A, Aznar J
Dept. Clinical Pathology, Hospital La Fe, Valencia, Spain.
Clin Hemorheol Microcirc. 1998 Jul;18(4):253-8.
In order to ascertain whether the increased aggregability observed in the red blood cells of diabetic patients is induced exclusively by plasma factors or is also influenced by membrane lipids, we examined the phospholipids of the erythrocyte membrane, the plasma fibrinogen concentration and erythrocyte aggregation in 86 insulin and non insulin-dependent diabetic patients. The data obtained show that the erythrocyte aggregability of the diabetic patients is higher than that of the control group (10.0+/-2.4 vs. 7.8+/-1.6%). This increased aggregability correlates not only with a higher fibrinogen concentration but also with changes observed in the membrane phospholipids. The percentage of sphingomyelin (SP) in the patients is higher than in the controls (22.6+/-6.8 vs. 18.4+/-5.4%) and that of phosphatidylserine (PS) is lower (9.5+/-6.1 vs. 12.1+/-5.1%). No differences in the percentages of the other two phospholipids identified (phosphatidylcholine, PC, and phosphatidylethanolamine, PE) were observed. The increase in the saturated nature of the phospholipids of the erythrocyte membrane, which can be measured by the (SP + PC)/(PE + PS) ratio, is statistically related (r = 0.39; p < 0.05) to the increased red blood cell aggregability observed in these patients.
为了确定糖尿病患者红细胞中观察到的聚集性增加是仅由血浆因子诱导，还是也受膜脂质影响，我们检测了86例胰岛素依赖型和非胰岛素依赖型糖尿病患者红细胞膜的磷脂、血浆纤维蛋白原浓度和红细胞聚集性。所得数据表明，糖尿病患者的红细胞聚集性高于对照组（10.0±2.4对7.8±1.6%）。这种增加的聚集性不仅与较高的纤维蛋白原浓度相关，还与膜磷脂的变化相关。患者中鞘磷脂（SP）的百分比高于对照组（22.6±6.8对18.4±5.4%），而磷脂酰丝氨酸（PS）的百分比则较低（9.5±6.1对12.1±5.1%）。在另外两种已鉴定的磷脂（磷脂酰胆碱，PC，和磷脂酰乙醇胺，PE）的百分比上未观察到差异。红细胞膜磷脂饱和性的增加，可用（SP+PC）/（PE+PS）比值来衡量，与这些患者中观察到的红细胞聚集性增加具有统计学相关性（r = 0.39；p < 0.05）。
