Eshet R, Assa S, Laron Z
Biol Neonate. 1976;29(5-6):354-9. doi: 10.1159/000240885.
Fetal and adult human growth hormone (HGH) extract from human pituitaries as well as plasma from premature and full-term newborns and children were submitted to chromatography on Sephadex G-75 and the immunoreactive HGH (IRHGH) in the elution fractions was determined quantitatively by radioimmunoassay. Three fractions of IRHGH could be differentiated according to the elution pattern: 'big-big', 'big' and 'little' IRHGH. In fetal pituitary extract only 'big-big' and 'little' IRHGH were found, whereas in adult pituitary extract all three forms of IRHGH were present. In the plasma samples studied no 'big-big' form was found; only 'big' and 'little' IRHGH appeared. In addition, plasma samples of premature newborns had a smaller proportion of the 'big' IRHGH than did those of the full-term newborns and older children.
将从人垂体中提取的胎儿及成人的人类生长激素（HGH），以及早产和足月新生儿及儿童的血浆，进行Sephadex G - 75柱色谱分析，并通过放射免疫测定法定量测定洗脱组分中的免疫反应性HGH（IRHGH）。根据洗脱模式可区分出三种IRHGH组分：“大大”、“大”和“小”IRHGH。在胎儿垂体提取物中仅发现“大大”和“小”IRHGH，而在成人垂体提取物中三种形式的IRHGH均存在。在所研究的血浆样本中未发现“大大”形式；仅出现“大”和“小”IRHGH。此外，早产新生儿血浆样本中“大”IRHGH的比例低于足月新生儿和大龄儿童的血浆样本。
