Sándor E, Pusztahelyi T, Karaffa L, Karányi Z, Pócsi I, Biró S, Szentirmai A, Pócsi I
Department of Microbiology and Biotechnology, Kossuth Lajos University, Debrecen, Hungary.
FEMS Microbiol Lett. 1998 Jul 15;164(2):231-6. doi: 10.1111/j.1574-6968.1998.tb13091.x.
The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate.
假三糖别藻氨糖是一种几丁质酶的有效抑制剂，它能延缓产黄顶头孢霉菌丝的断裂，但不影响其真菌生长和头孢菌素C的产生。别藻氨糖对产黄顶头孢霉细胞结合几丁质酶的体外抑制作用表明，约47%的可溶性细胞内几丁质酶活性对别藻氨糖的抑制作用具有抗性。另一方面，别藻氨糖能有效抑制可溶性和不溶性酶组分中总几丁质酶活性的约76%。所有几丁质酶活性均采用一种基于纯化的产黄顶头孢霉几丁质作为底物的新方法进行测定。
