Suzuki Shigeo, Nakanishi Eiyu, Furihata Keiko, Miyamoto Katsushiro, Tsujibo Hiroshi, Watanabe Takeshi, Ohnishi Yasuo, Horinouchi Sueharu, Nagasawa Hiromichi, Sakuda Shohei
Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Int J Biol Macromol. 2008 Jul 1;43(1):13-9. doi: 10.1016/j.ijbiomac.2007.09.010. Epub 2007 Sep 29.
Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R and chi65S, which were present at the 5'-upstream region of chi65. In this study, we showed generality of the allosamidin's effect. Allosamidin enhanced production of the family 18 chitinases originated from chi65h of Streptomyces halstedii MF425, another allosamidin producer, chiC of Streptomyces coelicolor A3(2) and chiIII of Streptomyces griseus. All the three chitinase genes had high homology to chi65 and two genes homologous to chi65S and chi65R were present at their 5'-upstream regions. When allosamidin's effect was tested with six Streptomyces strains randomly isolated from soil, allosamidin enhanced chitinase production of all strains. All six strains possessed a set of three genes homologous to chi65, chi65S and chi65R. Analysis of 16S rDNA indicated that allosamidin-sensitive strains are distributed widely in Streptomyces. These observations suggested that allosamidin can affect the common regulatory system for production of a chitinase with a two-component regulatory system in Streptomyces.
别洛沙米定是一种由链霉菌产生的18家族几丁质酶抑制剂。在其产生菌链霉菌AJ9463中，别洛沙米定通过chi65R和chi65S编码的双组分调控系统促进几丁质培养基中源自chi65的18家族几丁质酶的产生，chi65R和chi65S位于chi65的5'-上游区域。在本研究中，我们展示了别洛沙米定作用的普遍性。别洛沙米定增强了源自哈氏链霉菌MF425（另一种别洛沙米定产生菌）的chi65h、天蓝色链霉菌A3(2)的chiC和灰色链霉菌的chiIII的18家族几丁质酶的产生。所有这三个几丁质酶基因与chi65具有高度同源性，并且在它们的5'-上游区域存在两个与chi65S和chi65R同源的基因。当用从土壤中随机分离的六种链霉菌菌株测试别洛沙米定的作用时，别洛沙米定增强了所有菌株的几丁质酶产生。所有六种菌株都拥有一组与chi65、chi65S和chi65R同源的三个基因。16S rDNA分析表明，对别洛沙米定敏感的菌株广泛分布于链霉菌中。这些观察结果表明，别洛沙米定可以影响链霉菌中具有双组分调控系统的几丁质酶产生的共同调控系统。
