Aufurth S, Madkour M, Mayer F, Müller V
Lehrstuhl für Mikrobiologie der Ludwig-Maximilians-Universität München, Munich, Germany.
FEBS Lett. 1998 Sep 4;434(3):325-8. doi: 10.1016/s0014-5793(98)01008-4.
The Na+-dependent flagellum of Acetobacterium woodii was characterised. Flagellin and whole flagella were purified and analysed by SDS-PAGE and electron microscopy. The structure and dimensions of the filament and the hook-basal body, as revealed by electron microscopy, resemble those of H+-dependent flagella from gram-positive bacteria. Intramembrane particle rings were present at the cell pole in freeze-fractured A. woodii cells, which might correspond to the mot complex.
对伍氏醋杆菌依赖钠离子的鞭毛进行了表征。纯化了鞭毛蛋白和完整鞭毛,并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和电子显微镜进行分析。电子显微镜显示的丝状体以及钩形基体的结构和尺寸,类似于革兰氏阳性菌中依赖氢离子的鞭毛。在经冷冻蚀刻的伍氏醋杆菌细胞的细胞极处存在膜内颗粒环,其可能对应于运动复合体。