Miller D L, Dibbens J A, Damert A, Risau W, Vadas M A, Goodall G J
Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA, Australia.
FEBS Lett. 1998 Sep 4;434(3):417-20. doi: 10.1016/s0014-5793(98)01025-4.
Vascular endothelial growth factor (VEGF), an essential regulator of angiogenesis during early development as well as during the growth of solid tumours, bears an unusually large 5' untranslated region (5'-UTR) in the mRNA of over 1000 nucleotides. We found that the VEGF 5'-UTR, despite being GC-rich and containing an upstream short open reading frame, promotes efficient translation of a luciferase reporter. The VEGF 5'-UTR also allowed translation of luciferase from a dicistronic mRNA when placed between the two cistrons, demonstrating that it contains an internal ribosome entry site. Deletion analysis indicated that the IRES resides towards the 3' end of the 5'-UTR.
血管内皮生长因子（VEGF）是早期发育以及实体瘤生长过程中血管生成的关键调节因子，其mRNA具有一个超过1000个核苷酸的异常大的5'非翻译区（5'-UTR）。我们发现，VEGF的5'-UTR尽管富含GC且包含一个上游短开放阅读框，但仍能促进荧光素酶报告基因的高效翻译。当VEGF的5'-UTR置于双顺反子mRNA的两个顺反子之间时，它也能使荧光素酶从双顺反子mRNA中翻译出来，这表明它含有一个内部核糖体进入位点。缺失分析表明，内部核糖体进入位点（IRES）位于5'-UTR的3'端附近。
