Chaitidis P, Schewe T, Sutherland M, Kühn H, Nigam S
Department of Gynaecology, University Medical Centre Benjamin Franklin, Free University Berlin, Germany.
FEBS Lett. 1998 Sep 4;434(3):437-41. doi: 10.1016/s0014-5793(98)01024-2.
Reticulocyte-type 15-lipoxygenase is known to dioxygenate phospholipids without preceding action of phospholipases A2 (PLA2). Therefore we studied the reaction of the secretory PLA2s (sPLA2) from pancreas and snake venom, and of the human cytosolic PLA2 (cPLA2) with 1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC) and their 15-lipoxygenated species (PAPC-OOH and PAPC-OH) either alone or as equimolar mixtures. These PLA2s cleaved PAPC-O(O)H with higher (sPLA2) or similar rates (cPLA2) as compared with native PAPC. In mixtures, however, PAPC proved to be the preferred, albeit not exclusive substrate for all three PLA2s. Thus, partial 15-lipoxygenation of phospholipids may also trigger liberation of arachidonic acid.
已知网织红细胞型15-脂氧合酶在磷脂酶A2（PLA2）无预先作用的情况下对磷脂进行双加氧反应。因此，我们研究了胰腺和蛇毒分泌型PLA2（sPLA2）以及人胞质PLA2（cPLA2）与1-棕榈酰-2-花生四烯酰磷脂酰胆碱（PAPC）及其15-脂氧合产物（PAPC-OOH和PAPC-OH）单独或作为等摩尔混合物的反应。与天然PAPC相比，这些PLA2以更高的速率（sPLA2）或相似的速率（cPLA2）裂解PAPC-O（O）H。然而，在混合物中，PAPC被证明是所有三种PLA2的首选底物，尽管不是唯一的底物。因此，磷脂的部分15-脂氧合也可能引发花生四烯酸的释放。
