Sévenier R, Hall R D, van der Meer I M, Hakkert H J, van Tunen A J, Koops A J
Department of Cell Biology, Centre for Plant Breeding and Reproduction Research, Agricultural Research Department (CPRO-DLO), Wageningen, The Netherlands.
Nat Biotechnol. 1998 Sep;16(9):843-6. doi: 10.1038/nbt0998-843.
We have transformed sugar beet into a crop that produces fructans. The gene encoding 1-sucrose:sucrose fructosyl transferase (1-SST), which was isolated from Helianthus tuberosus, was introduced into sugar beet. In H. tuberosus, 1-SST mediates the first steps in fructan synthesis through the conversion of sucrose (GF) into low molecular weight fructans GF2, GF3, and GF4. In the taproot of sugar beet transformed with the 1-sst gene, the stored sucrose is almost totally converted into low molecular weight fructans. In contrast, 1-sst expression in the leaves resulted in only low levels of fructans. Despite the storage carbohydrate having been altered, the expression of the 1-sst gene did not have any visible effect on phenotype and did not affect the growth rate of the taproot as observed under greenhouse conditions.
我们已将甜菜转化为一种能产生果聚糖的作物。从菊芋中分离出的编码1-蔗糖：蔗糖果糖基转移酶（1-SST）的基因被导入甜菜。在菊芋中，1-SST通过将蔗糖（GF）转化为低分子量果聚糖GF2、GF3和GF4介导果聚糖合成的第一步。在用1-sst基因转化的甜菜主根中，储存的蔗糖几乎完全转化为低分子量果聚糖。相比之下，1-sst在叶片中的表达仅导致果聚糖水平较低。尽管储存碳水化合物发生了改变，但在温室条件下观察到，1-sst基因的表达对表型没有任何可见影响，也不影响主根的生长速率。
