Hasegawa H, Nomura T, Kishimoto K, Yanagisawa K, Fujita S
First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Japan.
J Immunol. 1998 Sep 15;161(6):3087-95.
In this study we have analyzed the adhesion molecules associated with and the biologic function of SFA-1/PETA-3 (CD151) in human T cell leukemia virus type 1 (HTLV-1)-infected T cells and in freshly isolated adult T cell leukemia (ATL) cells using an anti-CD151 mAb. The anti-CD151 mAb coprecipitated alpha 5 beta 1 integrin from HTLV-1-infected T cells. Conversely, an anti-alpha 5 integrin mAb coprecipitated CD151. The anti-CD151 mAb inhibited the adhesion of HTLV-1-infected T cells to fibronectin but did not have any effect on their adhesion to laminin, collagen type I, or collagen type IV. Moreover, antisense CD151 oligonucleotide-treated HTLV-1-infected T cells showed significant inhibition of adhesion to fibronectin. These findings showed that the CD151 molecule was associated with the alpha 5 beta 1 integrin molecule and that it enhanced alpha 5 beta 1 integrin-mediated adhesion to fibronectin. In addition, the expression levels of CD151, alpha 4 beta 1 integrin, and alpha 5 beta 1 integrin on ATL cells from lymph nodes of lymphoma-type ATL patients were significantly higher than those on circulating ATL cells from leukemia-type ATL patients. This suggests that the increased expression of these integrins may contribute to lymphoma formation through the adhesion of ATL cells to the extracellular matrix and dendritic cells, rather than contributing to transmigration.
在本研究中，我们使用抗CD151单克隆抗体，分析了与1型人类T细胞白血病病毒（HTLV-1）感染的T细胞以及新鲜分离的成人T细胞白血病（ATL）细胞中SFA-1/PETA-3（CD151）相关的黏附分子及其生物学功能。抗CD151单克隆抗体从HTLV-1感染的T细胞中共沉淀出α5β1整合素。相反，抗α5整合素单克隆抗体共沉淀出CD151。抗CD151单克隆抗体抑制HTLV-1感染的T细胞与纤连蛋白的黏附，但对它们与层粘连蛋白、I型胶原或IV型胶原的黏附没有任何影响。此外，经反义CD151寡核苷酸处理的HTLV-1感染的T细胞对纤连蛋白的黏附显示出显著抑制。这些发现表明，CD151分子与α5β1整合素分子相关，并且它增强了α5β1整合素介导的对纤连蛋白的黏附。此外，淋巴瘤型ATL患者淋巴结中ATL细胞上CD151、α4β1整合素和α5β1整合素的表达水平显著高于白血病型ATL患者循环中的ATL细胞。这表明这些整合素表达的增加可能通过ATL细胞与细胞外基质和树突状细胞的黏附促进淋巴瘤的形成，而不是促进迁移。
