Liossis S N, Hoffman R W, Tsokos G C
Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, 20814, USA.
Clin Immunol Immunopathol. 1998 Sep;88(3):305-10. doi: 10.1006/clin.1998.4569.
Multiple immunoregulatory abnormalities characterize systemic lupus erythematosus. Abnormalities of the antigen receptor-mediated early signal transduction biochemical events underscore the diverse cellular aberrations. Fresh peripheral T and B cells and T cell lines from patients with systemic lupus erythematosus display increased Ca2+ responses that are preceded by enhanced antigen receptor-initiated cytosolic protein tyrosine phosphorylation. To further dissect the aberrant signaling events of lupus T cells we studied the early anti-CD3 mAb-induced signaling events in autoantigen-specific T cells from lupus patients. We report herein that a lupus snRNP-specific T cell clone, but not other T cells, displays increased Ca2+ fluxes and enhanced production of tyrosine-phosphorylated proteins following TCR/CD3 stimulation.
多种免疫调节异常是系统性红斑狼疮的特征。抗原受体介导的早期信号转导生化事件异常突出了多种细胞异常。系统性红斑狼疮患者的新鲜外周T细胞和B细胞以及T细胞系显示出Ca2+反应增加，这之前抗原受体启动的胞质蛋白酪氨酸磷酸化增强。为了进一步剖析狼疮T细胞的异常信号事件，我们研究了狼疮患者自身抗原特异性T细胞中早期抗CD3单克隆抗体诱导的信号事件。我们在此报告，一个狼疮snRNP特异性T细胞克隆，而非其他T细胞，在TCR/CD3刺激后显示出增加的Ca2+通量和酪氨酸磷酸化蛋白产量增加。
