Srikumar T S, Wezendonk B, van Dokkum W
Department of Biochemistry, Faculty of Medicine, Kuwait University, Safat, Kuwait.
Ann Nutr Metab. 1998;42(4):231-6. doi: 10.1159/000012738.
In the present pilot study, for investigating the physiological effects of different types of nondigestible oligosaccharides, we have validated the application of methodologies for the analysis of bile acids and neutral steroids in feces of human subjects. The accuracy of the extraction and chromatographic procedures for the analyses of bile acids and neutral steroids was determined by recovery of added compounds to fecal homogenate. The precision of the above procedures was checked by analyzing these compounds in samples (n = 5) of the same fecal homogenate. Recoveries of added bile acids ranged from 86 and 96%, and those of neutral steroids varied from 81 to 97%. The precision expressed as coefficients of variation of bile acids and neutral steroids ranged from 2.3 to 8.3% and from 6.3 to 11.8%, respectively. The intra- and interindividual variabilities expressed as coefficients of variation of bile acids varied from 1 to 58 and from 0 to 74%, respectively. The same variabilities for neutral steroids ranged from 0.5 to 107% and from 1 to 168%, respectively. The methods validated in the present pilot study were adequate for applying to our forthcoming European Union coordinated major study on the physiological effects of different types of nondigestible oligosaccharides and involving large numbers of samples.
在本初步研究中，为了探究不同类型的不可消化寡糖的生理效应，我们已经验证了用于分析人类受试者粪便中胆汁酸和中性类固醇的方法的适用性。通过将添加化合物回收到粪便匀浆中来确定胆汁酸和中性类固醇分析的提取和色谱程序的准确性。通过分析同一粪便匀浆的样本（n = 5）中的这些化合物来检查上述程序的精密度。添加胆汁酸的回收率在86%至96%之间，中性类固醇的回收率在81%至97%之间。以胆汁酸和中性类固醇变异系数表示的精密度分别在2.3%至8.3%和6.3%至11.8%之间。以胆汁酸变异系数表示的个体内和个体间变异性分别在1%至58%和0%至74%之间。中性类固醇的相同变异性分别在0.5%至107%和1%至168%之间。在本初步研究中验证的方法适用于我们即将开展的欧盟协调的关于不同类型不可消化寡糖生理效应的大型主要研究，该研究涉及大量样本。
