Chang B V, Su C J, Yuan S Y
Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China.
Chemosphere. 1998 Jun;36(13):2721-30. doi: 10.1016/s0045-6535(97)10231-4.
The potential dechlorination of hexachlorobenzene (HCB) in medium by 1,2,3-trichlorobenzene (TCB)-adapted mixed culture under three reducing conditions was investigated. It was found that strongest to weakest HCB dechlorination occurred in the order of methanogenic conditions > sulfate-reducing conditions > denitrifying conditions. Under denitrifying conditions, no dechlorination was observed during the first 20 days of incubation. Biotransformation occurred in this order: HCB-->pentachlorobenzene (PCB)-->1,2,3,5-tetrachlorobenzene (TeCB)-->1,3,5-TCB + 1,2,4-TCB-->1,3-dichlorobenzene (DCB), HCB dechlorination was delayed following treatment with ferric chloride and manganese dioxide, but enhanced by the addition of lactate and pyruvate under methanogenic or sulfate-reducing conditions, the addition of acetate had no significant effect on HCB dechlorination under any of the three reducing conditions. Sequential dechlorination was observed at concentrations of 2-50 mg/L, but at a significantly slower rate at the highest concentrations.
研究了在三种还原条件下，适应1,2,3-三氯苯（TCB）的混合培养物对介质中六氯苯（HCB）的潜在脱氯作用。结果发现，HCB脱氯能力从强到弱依次为产甲烷条件＞硫酸盐还原条件＞反硝化条件。在反硝化条件下，培养的前20天未观察到脱氯现象。生物转化按此顺序发生：HCB→五氯苯（PCB）→1,2,3,5-四氯苯（TeCB）→1,3,5-TCB + 1,2,4-TCB→1,3-二氯苯（DCB），用氯化铁和二氧化锰处理后HCB脱氯延迟，但在产甲烷或硫酸盐还原条件下添加乳酸盐和丙酮酸盐可增强脱氯效果，在三种还原条件中的任何一种下添加乙酸盐对HCB脱氯均无显著影响。在2-50 mg/L的浓度下观察到顺序脱氯，但在最高浓度下脱氯速率明显较慢。
