Barceló F, Gutiérrez F, Barjau I, Portugal J
Departament de Biologia Fundamental i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
J Biomol Struct Dyn. 1998 Aug;16(1):41-50. doi: 10.1080/07391102.1998.10508225.
The curvature patterns of seven satellite DNAs taken from beetles belonging to the family Tenebrionidae (Coleoptera) were modelled utilising a number of computer programs that describe and plot the curvature profiles of DNA. The theoretical analysis agreed with the experimentally observed curvature of most of these satellite DNAs, and its absence in Tribolium freemani and Tenebrio obscurus satellite I. In many cases, the tenebrionid satellite DNAs lack periodically repeated runs of phased-A-tracts, yet they represent a clear example of curved DNA. The macroscopic curvature of satellites from these closely related organisms confirmed that other sequence elements must be participating in the bending of these DNAs. Our modelling approaches are discussed, together with previous experimental results, in terms of the role played by DNA curvature in the organisation of satellite DNA and the tight compacting of heterochromatin.
利用一些描述和绘制DNA曲率图谱的计算机程序，对从拟步甲科（鞘翅目）甲虫中提取的7种卫星DNA的曲率模式进行了建模。理论分析与这些卫星DNA中大多数的实验观察到的曲率一致，而在弗里曼拟谷盗和暗黑拟步甲卫星I中则没有观察到这种曲率。在许多情况下，拟步甲科卫星DNA缺乏周期性重复的相位A序列，但它们却是弯曲DNA的一个明显例子。这些密切相关生物的卫星DNA的宏观曲率证实，其他序列元件必定参与了这些DNA的弯曲。我们结合之前的实验结果，讨论了我们的建模方法，涉及DNA曲率在卫星DNA组织和异染色质紧密压缩中所起的作用。
