Plohl M, Borstnik B, Ugarković D, Gamulin V
Department of Organic Chemistry and Biochemistry, Ruder Bosković Institute, Zagreb, Yugoslavia.
Biochimie. 1990 Sep;72(9):665-70. doi: 10.1016/0300-9084(90)90049-m.
Single satellite DNA constitutes about 50% of the Tenebrio molitor genome. Electrophoresis of 142 base pair long satellite monomers on nondenaturating polyacrylamide gel shows retarded mobility, a characteristic of fragments with sequence-induced DNA curvature. Migrational analysis of circularly permuted satellite monomers revealed the existence of 2 bend centers in the monomer sequence. We calculated the trajectory of DNA helix axis according to the algorithm of De Santis et al. This model predicts that T molitor naked satellite DNA forms a solenoid structure with left-handed superhelix. One turn of the superhelix has approximately 310 base pairs and a 33 nm pitch. Point mutations found in the satellite DNA (1.8%) influence bending characteristics, but do not distort the general geometry of satellite superhelix.
单卫星DNA约占黄粉虫基因组的50%。在非变性聚丙烯酰胺凝胶上对142个碱基对长的卫星单体进行电泳，显示迁移率延迟，这是具有序列诱导DNA弯曲的片段的特征。对环状排列的卫星单体进行迁移分析，揭示了单体序列中存在2个弯曲中心。我们根据De Santis等人的算法计算了DNA螺旋轴的轨迹。该模型预测，黄粉虫裸露的卫星DNA形成具有左手超螺旋的螺线管结构。超螺旋的一圈大约有310个碱基对，螺距为33纳米。在卫星DNA中发现的点突变（1.8%）影响弯曲特性，但不会扭曲卫星超螺旋的总体几何形状。
