Muramatsu T, Imai T, Park H M, Watanabe H, Nakamura A, Okumura J
Laboratory of Department of Biological Resources and Environmental Sciences, School of Agricultural Sciences, Nagoya University, Japan.
Mol Cell Biochem. 1998 Aug;185(1-2):27-32. doi: 10.1023/a:1016507900718.
In order to search tissue-specific elements in the 5'-upstream promoter region, gene gun was used to transfect in vivo plasmid DNAs with varying lengths of truncated ovalbumin promoter fused to the CAT reporter gene to the oviduct and liver of laying hens. The results indicated that in the oviduct, consistently high reporter gene expression was observed irrespective of the length of the truncated ovalbumin gene promoters, whereas in the liver the ovalbumin promoter extending from -3200 to +8 bp suppressed substantially the reporter gene expression compared with consistently high gene expression obtained by the ovalbumin promoters from -2800 to +8 bp or shorter length. It was concluded, therefore, that a tissue-specific silencer-like element might reside most likely in the ovalbumin gene promoter region between -3200 and -2800 bp which represses the ovalbumin gene transcription in the liver, but not in the oviduct of laying hens.
为了在5'-上游启动子区域寻找组织特异性元件，使用基因枪将与CAT报告基因融合的不同长度截短的卵清蛋白启动子的体内质粒DNA转染到产蛋母鸡的输卵管和肝脏中。结果表明，在输卵管中，无论截短的卵清蛋白基因启动子的长度如何，均观察到报告基因持续高表达，而在肝脏中，与从-2800到+8 bp或更短长度的卵清蛋白启动子获得的持续高基因表达相比，从-3200到+8 bp的卵清蛋白启动子显著抑制了报告基因表达。因此得出结论，组织特异性沉默子样元件最有可能存在于-3200至-2800 bp之间的卵清蛋白基因启动子区域，该区域抑制肝脏中卵清蛋白基因的转录，但不抑制产蛋母鸡输卵管中的转录。
